| 质粒名称: | pBS185 CMV-Cre |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11916 |
| 存储实验室: | Brian Sauer |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，Cre/Lox |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | cre |
| 物种来源: | bacteriophage P1 |
| 插入大小: | 1100 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBS185 CMV-Cre was a gift from Brian Sauer (Addgene plasmid # 11916 ; http://n2t.net/addgene:11916 ; RRID:Addgene_11916) |
| 参考文献部分: | Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. Sauer B, Henderson N. New Biol. 1990 May . 2(5):441-9. PubMed 2288914 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBS185 CMV-Cre 6919 bp ds-DNA circular SYN 06-FEB-2020
DEFINITION Mammalian expression of Cre recombinase driven by the human
cytomegalovirus IE promoter.
ACCESSION .
VERSION .
KEYWORDS pBS185 CMV-Cre
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6919)
AUTHORS Sauer B, Henderson N
TITLE Targeted insertion of exogenous DNA into the eukaryotic genome by
the Cre recombinase.
JOURNAL New Biol. 1990 May . 2(5):441-9.
PUBMED 2288914
REFERENCE 2 (bases 1 to 6919)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 6, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6919
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(565..581)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(565..581)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(578..600)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 589..605
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(613..643)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 658..679
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(796..813)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(967..1555)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1047..1066)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1726..2586)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2349..2368
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2587..2691)
/gene="bla"
/label=AmpR promoter
primer_bind 2759..2777
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2815..2837)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2937..2956
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3150..3172
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3164..3181
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3165..3181
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 3267..3646
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3647..3850
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3801..3821
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3847..3871
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3991..5022
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
RBS 6674..6682
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
1 cactattgca tatgccatca agattttgct gaaaggaccc tgataaagct gtctcctgtg
61 aggctttgcc agtgcccagc aaacacagaa gtggatgctc acagtcagct attggatgga
121 acacagggcc cccaatggag gagctagaga aagtacccaa ggagctaaag ggatctgcaa
181 ccctataggt ggaacagcaa tctgaactaa ccagtacccc ctgaactcgt gtatctagct
241 tcgtatgtag cagaagatgg cctagttggc catcactggg aagagaggac ccttggtcta
301 gcaaactata tgccccagtt caggggaaag ccagggccaa gaagtgggag tgagtgggta
361 ggggagcggg gtgggggtag gggtggggta taggggactt ttgggatagc atttgaaata
421 taaacgaaga aaatatccaa aaaaaaaaag atggaaaaaa aagtgataaa gagcgtgggt
481 cctgcctgta atcaagatat ttgagatgta gcagcaggaa ggccaggaat tggtcgaggg
541 taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
661 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
721 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1381 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
1441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
1561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
1621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
1681 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
1741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
1801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
1861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
1921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
1981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2041 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2341 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
2521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
2581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
2641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
2701 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
2761 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
2821 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
2881 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
2941 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
3001 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
3061 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
3121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
3181 tgccaagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
3241 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
3301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
3361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
3421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
3481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
3541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
3601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
3661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
3721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
3781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
3841 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
3901 cctccataga agacaccgac tctagaggat cccctcgagg ggcagagccg atcctgtaca
3961 ctttacttaa aaccattatc tgagtgtgaa atgtccaatt tactgaccgt acaccaaaat
4021 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg
4081 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg
4141 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat
4201 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa
4261 catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc
4321 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt
4381 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa
4441 aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc
4501 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt
4561 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag
4621 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta
4681 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca
4741 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga
4801 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt
4861 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg
4921 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt
4981 gaaacagggg caatggtgcg cctgctggaa gatggcgatt agccattaac gcgtaaatga
5041 ttgctataat tatttgatat ttatggtgac atatgagaaa ggatttcaac atcgacggaa
5101 aatatgtagt gctgtctgta agcactaata ttcagtcgcc agccgtcatt gtcactgtaa
5161 agctgagcga tagaatgcct gatattgact caatatccgt tgcgtttcct gtcaaaagta
5221 tgcgtagtgc tgaacatttc gtgatgaatg ccaccgagga agaagcacgg cgcggttttg
5281 ctaaagtgat gtctgagttt ggcgaactct tgggtaaggt tggaattgtc gagggatctc
5341 ggaatggacc ccaactgctc ctgctccacc ggtaagactc ccgatccttg gtctttagaa
5401 taccaagttg ggaccgcaga gcggaatccc cgagttgtag aggcttggcg ggaataggca
5461 cctttagttg gcgattcatt ccggttcttt ctagaatccg ctcttgcaaa agccttcatt
5521 agttacgagt attgtcgaac gggtcctttg gcggggttgg ggctaggatt tagacgcgca
5581 aatgtccggt tcctgatcac ccagttagtg gggacatctg ggttgagtcc caggcattac
5641 taaacttact gtgaattgct tgaattaaga aagaggtgaa ggacctttat gtcttgggac
5701 tcaaagacat aatccctgac ttaacctgtg aggagaaaag tggggctagg ctccctgcag
5761 ctccgaggag gacttagtga actgagccgg gactcgtggt gttggccact gctgtaatgc
5821 tgcctccctc atgctgtctt ctttctcctc cccaggcggc tcctgcactt gcaccagctc
5881 ctgcgcctgc aagaactgca agtgcacctc ctgcaagaag agtgagttgg gacaccttgg
5941 gtggcggcta aggctagggg cggggaactc ctacaaaact ggctctgaga aatgtccttt
6001 gcttcccgga ggccattgta ttgtctcggg gacagaacta tacagagaac tatttaaaaa
6061 aaccgaggtc ttctctgttg gggacaggaa gcagaggtct tcagccaggc tgacctcttc
6121 ctcctccttt ctaggctgct gctcctgctg tcccgtgggc tgctccaaat gtgcccaggg
6181 ctgtgtctgc aaaggcgccg cggacaagtg cacgtgctgt gcctgatgtg acgaacagcg
6241 ctgccaccac gtgtaaatag tatcggacca acccagcgtc ttcctataca gttccaccct
6301 gtttactaaa cccccgtttt ctaccgagta cgtgaataat aaaagcctgt ttgagtctaa
6361 ctctggtttt cttggtgtgg tttggcaata agaaactggg gtgacttgat agtctgggga
6421 tctggttttg gaccccctcg tgcctttacc tcccgcctct ggccctcaca gaggggtaat
6481 gtctttgggt aaagccaagc tatcccataa gcttcctcat ggaaaacagc tgtgtgcctg
6541 gtcaggtctt gtgttagggg aagggaggga gggagggtgg tcctggaaga cttgatgtcc
6601 catcctggaa gcacagaaaa tcttatcatt tatattctct ttggattctg gtttcctctc
6661 acattgttgg gtataaggag gtgggtatga aaattaacat agggtgtctc gtatgctagg
6721 cagggactaa cactgaacta gattccagag gggctttctt ggctgttttg acacaagttg
6781 cttaccttgt cctagaactc attctgcagt ccagtctgac cttgagtttt tgattctcct
6841 gcctcagctt cccaagtacc tgggattaca ggagtgactg tgtcaccagg ccatctccta
6901 ttcagccacc aaatgcaga
//
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